Figure 3.
Figure 3. The regulatory role of Rer1p in cilia formation is conserved in mammalian cells. (a) Anti–acetylated tubulin staining and scanning EM demonstrates shorter cilia after rer1 knockdown in CL4 cells. (b) Efficiency of rer1 knockdown by RNAi (siRer1) in CL4 cells compared with nonspecific control (NSC) using at least three Western blots (normalized to GAPDH). (c) Unaffected number of ciliated cells upon rer1 knockdown in CL4. (d) Twofold decrease in mean cilia length upon rer1 knockdown in CL4 (n > 684). (e) Shorter cilia after rer1 down-regulation in RPE cells are rescued upon reexpression of HA-tagged Rer1p. Western blot is shown in g. Quantification (n ≥ 95) shown in f. Means ± SEM (d and f) or SD (b). ***, P < 0.001, Wilcoxon rank sum test.

The regulatory role of Rer1p in cilia formation is conserved in mammalian cells. (a) Anti–acetylated tubulin staining and scanning EM demonstrates shorter cilia after rer1 knockdown in CL4 cells. (b) Efficiency of rer1 knockdown by RNAi (siRer1) in CL4 cells compared with nonspecific control (NSC) using at least three Western blots (normalized to GAPDH). (c) Unaffected number of ciliated cells upon rer1 knockdown in CL4. (d) Twofold decrease in mean cilia length upon rer1 knockdown in CL4 (n > 684). (e) Shorter cilia after rer1 down-regulation in RPE cells are rescued upon reexpression of HA-tagged Rer1p. Western blot is shown in g. Quantification (n ≥ 95) shown in f. Means ± SEM (d and f) or SD (b). ***, P < 0.001, Wilcoxon rank sum test.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal