ATP hydrolysis mutant Arp2/3 complex produces dendritic actin network disassembly defects. This is demonstrated by the longer lifetimes and greater distances traveled of Arp2/3 speckles, as compared with WT Arp2/3. (A) We created the following Drosophila S2 stable cell lines: Arp2(WT)-GFP (3); Arp2Q137A-GFP (4); Arp3(WT)-GFP (6); and Arp3Q137A-GFP (7) (see Table 1 for complete list of cell lines). We imaged these stable cell lines and created kymographs from the movies (B–E, second column). We quantified the speckle distances traveled and speckle lifetimes. Endogenous Arp2 (B and C) and Arp3 (D and E) were depleted by dsRNA directed against the 5′ and 3′ UTRs, leaving Arp2-GFP (B and C) and Arp3-GFP (D and E) as the sole copy of Arp2 (B and C) and Arp3 (D and E) in the cell. Bar, 10 µm. (F) We replotted our speckle lifetime and distance-traveled measurements using a box-and-whisker plot. We used the nonparametric Kolmogorov-Smirnov test to compare ATP hydrolysis mutant data against WT data. We calculated p-values to be less than 0.001. The central mark in the box represents the median value; the edges of the box represent the 25th and 75th percentiles. Whiskers extend to the most extreme data points not considered outliers, and outliers are plotted individually, in red.