Figure 2.
Figure 2. Double-stranded RNA (dsRNA) directed against the 5′ and 3′ untranslated regions (UTRs) of ARP2 or ARP3 depletes the targeted endogenous protein, producing the serrated phenotype. Arp2-GFP and Arp3-GFP each rescue cells depleted of endogenous Arp2 and Arp3, respectively. We created stable S2 cells lines that express actin-GFP, either alone or with Arp2-mCherry (or Arp3-mCherry). dsRNA targeting the coding sequence of ARP2 (or ARP3) depletes the corresponding protein, resulting in the serrated phenotype (B, E, H, and K). In contrast, cells transfected with dsRNA targeting the 5′ and 3′ UTRs of ARP2 or ARP3 are selectively depleted of endogenous Arp2 or Arp3 (C and I), and are rescued by exogenously expressed Arp2-mCherry (F) and Arp3-mCherry (L). Bar, 10 µm.

Double-stranded RNA (dsRNA) directed against the 5′ and 3′ untranslated regions (UTRs) of ARP2 or ARP3 depletes the targeted endogenous protein, producing the serrated phenotype. Arp2-GFP and Arp3-GFP each rescue cells depleted of endogenous Arp2 and Arp3, respectively. We created stable S2 cells lines that express actin-GFP, either alone or with Arp2-mCherry (or Arp3-mCherry). dsRNA targeting the coding sequence of ARP2 (or ARP3) depletes the corresponding protein, resulting in the serrated phenotype (B, E, H, and K). In contrast, cells transfected with dsRNA targeting the 5′ and 3′ UTRs of ARP2 or ARP3 are selectively depleted of endogenous Arp2 or Arp3 (C and I), and are rescued by exogenously expressed Arp2-mCherry (F) and Arp3-mCherry (L). Bar, 10 µm.

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