The SAGA and THO/TREX component ENY-2 is required for peripheral positioning of hsp-16.2 promoter. (A) Sketch of budding yeast SAGA and THO/TREX complexes with yeast names (García-Oliver et al., 2012). The yeast subunit Sus1 (ENY-2, formerly e(y)2 in flies and T01C3.2 in worms) is present in both complexes (blue circle) and binds the nuclear basket. Other equivalents are Xmas-2 (ScSac3) and DSS1 (ScSem1). (B) Quantitation of hsp-16.2 transgene 1 position in progeny from control RNAi or eny-2(RNAi) fed adults, scored before and after HS. Scoring, zone 1, asterisks, and hatched bars are as in Fig. 2 (B and C). Loci counted were (left to right) n = 681, 704, 157, and 157. P-values versus random = 3 × 10−4, 3 × 10−7, 0.65, and 0.55, respectively. (C) Peripheral position of hsp-16.2 promoter tg #1 is unaffected by sams-3 RNAi, unlike the heterochromatic myo-3 array, GW76. GFP-lacI signals were scored in progeny of control RNAi or sams-3 RNAi fed worms. Scoring, zone 1 plotting, and hatching are as in Fig. 2 (B and C). Loci counted were (left to right) n = 681, 704, 298, and 270. P-values versus random = 3 × 10−4, 3 × 10−7, 2 × 10−8, and 2 × 10−13, respectively.
