Defective Alp4 telomere localization in haploid mto1Δ and dlc1Δ mutants and the telomere clustering mechanism. (A) Representative Alp4 localization in relation to telomeres (Taz1) and the SPB (Sid4). White lines indicate cell shapes. (B) Telomere (Telo) clustering in haploid meiotic cells (top graphs) and Alp4 telomere localization in MBC-treated cells (bottom graphs). The experiment of mto1Δ mutant was completed once. The data of other strains are from a single experiment out of two (wild type [Wt] and dhc1Δ) or three (dlc1Δ and dhc1Δ dlc1Δ) repeats. More than 100 cells were examined at each time point. (C) A proposed model for the telomere clustering mechanism. MT, microtubule.