Telomere clustering depends on microtubules. (A) The dynamics of telomeres (Telo) and microtubules (Mt) before cell conjugation. A left photo shows an examined cell, which is undergoing cell conjugation (white dashed box). (B) Schematic showing mat gene-induced haploid meiosis. (C) Meiotic progression (top graphs) and telomere clustering (bottom graphs) in haploid meiosis. Arrows show the addition of DMSO (left) or 50 µg/ml MBC (right). The data shown are from a single representative experiment out of two repeats. More than 100 cells were examined at each time point. Photos indicate the location of telomeres (Taz1) and SPB (Sid4). nuc, nucleus. (D and E) Dynamics of microtubules and telomeres in a haploid meiotic cell after MBC removal. MBC was removed ∼5 h after nitrogen depletion. In E, enlarged images are shown at the bottom row. Arrows show the telomere movement. White lines indicate cell shapes.