The immobility of Chs2 and Hof1 depends on a small region near the C-terminal end of Myo1. (A and B) Chs2 and Hof1 become mobile at the division site during cytokinesis in myo1-(AA1798Stop) cells. Chs2-GFP in strain YEF6771 (myo1-(AA1798Stop) CHS2-GFP CDC3-RFP; A) and Hof1-GFP in strain YEF6769 (myo1-(AA1798Stop) HOF1-GFP CDC3-RFP; B) were analyzed by FRAP during cytokinesis. Arrowheads denote bleaching events. Solid and dashed lines indicate the intensity measurements for the unbleached and bleached areas, respectively.