Proteins involved in septum formation or its coordination with the AMR display Myo1-dependent immobility during cytokinesis. (A–D) The immobility of Mlc1, Iqg1, Inn1, and Hof1 during cytokinesis depends on Myo1. FRAP analysis was performed on the indicated proteins in cells undergoing cytokinesis of the following strains: (A) YEF6065 (CDC3-RFP, pUG34-MLC1; left) and YEF6351 (myo1Δ CDC3-RFP, pUG34-MLC1; right); (B) YEF6140 (CDC3-RFP, pUG35-IQG1; left) and YEF6356 (myo1Δ CDC3-RFP, pUG35-IQG1; right, full-ring bleaching); (C) YEF6138 (INN1-GFP CDC3-RFP; left) and YEF6357 (myo1Δ INN1-GFP CDC3-RFP; n = 5; right); and (D) YEF6131 (HOF1-GFP CDC3-RFP; left) and YEF6358 (myo1Δ HOF1-GFP CDC3-RFP; right). Arrowheads denote additional half-ring or full-ring bleaching. WT, wild type.