Chs2 displays biphasic dynamics during cytokinesis, and its immobility depends on Myo1. (A) Chs2 is dynamic and then immobile during cytokinesis. Chs2-GFP in the strain YEF5874 (CHS2-GFP CDC3-RFP) was bleached sequentially during cytokinesis. Time 0 corresponds to the initial localization of Chs2 to the bud neck. Arrowheads denote bleaching events. Solid and dashed lines indicate the intensity measurements for the unbleached and bleached areas, respectively. (B) Chs2 immobility depends on Myo1. Chs2-GFP in the strain YEF6336 (myo1Δ CHS2-GFP CDC3-RFP) was subjected to FRAP analysis. Note that the duration of Chs2 at the bud neck was shorter, and the Chs2 signal was weaker in the myo1Δ strain than in the wild-type (WT) strain. (C and D) Myo1 immobility during cytokinesis does not depend on Chs2 and Inn1. Myo1-GFP in strains YEF6273 (chs2Δ MYO1-GFP CDC3-RFP) and YEF6230 (inn1Δ MYO1-GFP CDC3-RFP) was analyzed by FRAP.