Actin filaments are not required for Myo1 immobility during cytokinesis. (A) Myo1 remains immobile during cytokinesis in bni1Δ cells. Myo1-GFP ring in a cell of the strain YEF6116 (bni1Δ MYO1-GFP CDC3-RFP) undergoing cytokinesis was analyzed by FRAP. (B–E) Myo1-GFP displays similar dynamics in LatA- and DMSO-treated cells. Cells of the strain XDY286 (MYO1-GFP CDC3-RFP) were subjected to FRAP analysis in the presence LatA (B–D) or DMSO (E, control) during the indicated stages of the cell cycle. WT, wild type.