Periodically protruding cells can migrate. Left panels show start positions (white outline) and final positions of migrating cells and paths of cell movement (yellow). The separate images in the right panels illustrate the coordination between periodic protrusion and cell translocation for one oscillation period. (A) Merged DIC and Lifeact-GFP fluorescent images of rounded CHO cells without artificially depolymerized microtubules plated on collagen (10 mg/ml). Net translocation is 22 µm. (B) DIC images of rounded MDA-MB cells plated on glass with depolymerized microtubules. Total path is 92 µm and net translocation is 62 µm. (C) DIC images of rounded NIH 3T3 cells plated on glass with depolymerized microtubules. Total path is 108 µm and net translocation is 70 µm.