Cytoskeletal dynamics during periodic protrusions. (A) DIC and Lifeact-GFP wide-field fluorescent images of an oscillating cell. (B) Cytoskeletal dynamics during one period of oscillation (Video 1). Merged fluorescence images of highly polarized F-actin (green) and myosin II (red) throughout the oscillatory cycle. (C) Laser scanning confocal images and corresponding plots of the fluorescent signal from F-actin (Lifeact-GFP) of one representative oscillating cell (n = 35) for two time points. (left) Green rectangles (8.5 × 1.5 µm) show the positions where the fluorescent signal was analyzed. (right) Plots of the mean intensity for the corresponding fluorescent signal going from the inside to the outside of the cell. Bars, 5 µm; time shown in seconds.