Figure 2.
Figure 2. Cdc42 depletion does not affect Rac1 activation levels at nascent phagosomes. (A and B) Rac1 activity at the site of particle binding is not affected by Cdc42 depletion. Control (shScr) and Cdc42 (shCdc42)-depleted COS1 cells, expressing mCFP-Rac1 and PBD-mYFP were incubated with Y. pseudotuberculosis or 4.5-µm invasin-coated beads. Each condition was imaged microscopically for FRET analysis. Arrowheads denote regions of particle binding. Bar, 20 µm. (C and D) Quantification of A and B. The FRET signal at the site of particle attachment was quantified for each scenario. Error bars represent SEM.

Cdc42 depletion does not affect Rac1 activation levels at nascent phagosomes. (A and B) Rac1 activity at the site of particle binding is not affected by Cdc42 depletion. Control (shScr) and Cdc42 (shCdc42)-depleted COS1 cells, expressing mCFP-Rac1 and PBD-mYFP were incubated with Y. pseudotuberculosis or 4.5-µm invasin-coated beads. Each condition was imaged microscopically for FRET analysis. Arrowheads denote regions of particle binding. Bar, 20 µm. (C and D) Quantification of A and B. The FRET signal at the site of particle attachment was quantified for each scenario. Error bars represent SEM.

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