Figure 3.
Figure 3. Endogenous EFR3 and TTC7 are required for recruitment of GFP-M1-PI4KIIIα to the plasma membrane. HeLa cells were treated with a negative control siRNA or siRNA against EFR3A and EFR3B (siEFR3) and TTC7A and TTC7B (siTTC7) either alone or in combination. After 24 h, the cells were transfected with GFP-M1*-PI4KIIIα or GFP-M1-PI4KIIIα, and after an additional 24 h, the cells were imaged by TIRF microscopy, with a 1-s interval in between each image acquisition. (A) Representative images for each of the samples quantified in B. (B) Quantification of the number of GFP puncta observed at the plasma membrane, normalized per unit area. Error bars indicate standard deviations. *, P < 0.005 and **, P < 0.02, relative to GFP-M1-PI4KIIIα with control siRNA (n = 4–6). Bar, 5 µm.

Endogenous EFR3 and TTC7 are required for recruitment of GFP-M1-PI4KIIIα to the plasma membrane. HeLa cells were treated with a negative control siRNA or siRNA against EFR3A and EFR3B (siEFR3) and TTC7A and TTC7B (siTTC7) either alone or in combination. After 24 h, the cells were transfected with GFP-M1*-PI4KIIIα or GFP-M1-PI4KIIIα, and after an additional 24 h, the cells were imaged by TIRF microscopy, with a 1-s interval in between each image acquisition. (A) Representative images for each of the samples quantified in B. (B) Quantification of the number of GFP puncta observed at the plasma membrane, normalized per unit area. Error bars indicate standard deviations. *, P < 0.005 and **, P < 0.02, relative to GFP-M1-PI4KIIIα with control siRNA (n = 4–6). Bar, 5 µm.

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