Figure 7.
Figure 7. Mid1p-dependent and Cdc15p-dependent pathways are dispensable for actin filament assembly. (A) mid1-18, cdc15-140, and mid1-18 cdc15-140 cells expressing LAGFP and mCh-Atb2p were shifted to 36°C and imaged by spinning-disk microscopy at 36°C. mid1Δ and cdc15Δ cells expressing LAGFP and mCh-Atb2p were grown at 24°C and imaged by spinning-disk microscopy at 24°C. The cdc15Δ LAGFP mCh-Atb2p cell was germinated from spores. (B) Quantitation of the mutant cells in A with actin cables. Cells with spindles were grouped into two categories: short and long spindles. A single representative image from three independent experiments is shown. In each experiment, 100 cells were counted for each bar. Dashed line indicates the cell boundary. Bars, 5 µm.

Mid1p-dependent and Cdc15p-dependent pathways are dispensable for actin filament assembly. (A) mid1-18, cdc15-140, and mid1-18 cdc15-140 cells expressing LAGFP and mCh-Atb2p were shifted to 36°C and imaged by spinning-disk microscopy at 36°C. mid1Δ and cdc15Δ cells expressing LAGFP and mCh-Atb2p were grown at 24°C and imaged by spinning-disk microscopy at 24°C. The cdc15Δ LAGFP mCh-Atb2p cell was germinated from spores. (B) Quantitation of the mutant cells in A with actin cables. Cells with spindles were grouped into two categories: short and long spindles. A single representative image from three independent experiments is shown. In each experiment, 100 cells were counted for each bar. Dashed line indicates the cell boundary. Bars, 5 µm.

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