Synergistic growth acceleration and rescue induced by XMAP215^msps, EB1, and Sentin. (A) Kymographs showing MT polymerization dynamics with various combinations of EB1, Sentin, and XMAP215^msps in the presence of 15 µM tubulin. See also Video 1. (B) Parameters of MT polymerization dynamics. The mean values of each experiment are marked in gray, whereas the mean values of all the experiments have been indicated by black bars. The relative values are shown in these graphs, whereas the actual values are presented in Table S5. 400 nM EB1, 200 nM Sentin, and 100 nM XMAP215^msps-HA were added to tubulin. Some of the data (e.g., tubulin + XMAP215^msps-HA) overlapped with those in Figs. 2 and 3. (C) Mean catastrophe length in the presence or absence of EB1–Sentin or XMAP215^msps. The identical dataset was used as in B. The mean values of each experiment are marked in gray, whereas the mean values of all the experiments are indicated by black bars. The relative values are shown in these graphs, whereas the actual values are presented in Table S5. (D, left) MT rescue (arrows) in the presence of EB1, Sentin, and XMAP215^msps. The dashed lines indicate the ends of MT seeds. (right) XMAP215^msps- and Sentin-dependent increase in rescue frequency. In the presence of 15 µM tubulin, 400 nM EB1, and 200 nM Sentin (or 300 nM XMAP215^msps), various amounts of XMAP215^msps (or Sentin) were added. More rescue events were observed with increasing concentrations of Sentin and XMAP215^msps. The data shown are from a single representative experiment out of three repeats. Data from a single experiment are marked with the same shape. Bars: (horizontal) 5 µm; (vertical) 1 min.