Sentin accelerates MT growth and induces catastrophe in the presence of EB1. (A) Sentin recombinants used in this study. mGFP sequences were added to the N terminus. (B) Kymographs showing GFP-Sentin localization onto the growing MT plus ends in an EB1-dependent manner. Bars: (horizontal) 5 µm; (vertical) 1 min. (C) Parameters of MT polymerization dynamics in the presence of various concentrations of Sentin. Tubulin, 15 µM; EB1, 400 nM. The mean values of each experiment are marked in gray, whereas the mean values of all the experiments are indicated by black marks. The relative values are plotted in these graphs, whereas the actual values are presented in Table S4. Data from a single experiment are marked with the same shape. (D) Catastrophe length, the MT length at the time of catastrophe, was measured in the presence or absence of 400 nM EB1 and 200 nM Sentin. Tubulin was used at a 15-µM concentration. The frequency was plotted with SEM (n = 3 experiments; bin = 1 µm).