FtsZ1 and FtsZ2 filament morphologies in S. pombe single- and coexpression strains. (A and B, D and E, G–M) Epifluorescence micrographs of cells expressing FtsZ1-eYFP (A and B, J and K; green), FtsZ2-eCFP (D and E, L and M; red), FtsZ1-eYFP and FtsZ2-eCFP (G–I), eYFP (N), or eCFP (O). Because the eYFP signals in J and K or eCFP signals in L and M are from the same identically processed images, respectively, the differences in fluorescence intensity reflect differences in protein level. Dotted lines show cell outlines. (C and F) Differential interference contrast micrographs of cells in A and D, respectively. Bars, 5 µm.