Figure 4. Analysis of cell polarization... | Rockefeller University Press

Figure 4.

$Figure 4. Analysis of cell polarization orientations on stiff and soft substrates. (A) MCF10As transfected with Golgi-GFP undergoing wound healing on soft (3 kPa) versus stiff (65 kPa) substrates. Bar, 50 µm. (B) The orientations of Golgi relative to the direction of wound healing, as measured from centroids of cell nuclei, were plotted in rose plots at various time points after wounding for cells at the wound edge. The direction of each bar in the rose plots indicates the angular Golgi orientations, whereas the magnitude of each bar shows the fraction of cells with the indicated Golgi orientations. (C) Rose plots of Golgi orientations at 12 h after wounding for cells at various rows behind the wound edge cells. (D) The primary direction of lamellipodial protrusions in cells on soft (3 kPa) and stiff (65 kPa) substrates was quantified as in the direction of wound healing, opposite the direction of the wound, and toward other directions. Measurements were obtained for >30 cells at various distances from the wound edge from N experiments. Error bars show SEM.$

Analysis of cell polarization orientations on stiff and soft substrates. (A) MCF10As transfected with Golgi-GFP undergoing wound healing on soft (3 kPa) versus stiff (65 kPa) substrates. Bar, 50 µm. (B) The orientations of Golgi relative to the direction of wound healing, as measured from centroids of cell nuclei, were plotted in rose plots at various time points after wounding for cells at the wound edge. The direction of each bar in the rose plots indicates the angular Golgi orientations, whereas the magnitude of each bar shows the fraction of cells with the indicated Golgi orientations. (C) Rose plots of Golgi orientations at 12 h after wounding for cells at various rows behind the wound edge cells. (D) The primary direction of lamellipodial protrusions in cells on soft (3 kPa) and stiff (65 kPa) substrates was quantified as in the direction of wound healing, opposite the direction of the wound, and toward other directions. Measurements were obtained for >30 cells at various distances from the wound edge from N experiments. Error bars show SEM.