MT1-MMP is tethered to invasive pseudopods in an N-WASP–dependent manner. (A) MDA-MB-231 were transfected with mCherry-MT1-MMP or mCherry-MT1-MMPΔCT, as indicated, as well as GFP-actin, and subjected to CIA. Selected actin-rich areas in invasive pseudopods or areas of the cell body (boxes shown in A and quantified in B) of NT or N-WASP–depleted cells as indicated were photobleached using a 405-nm laser, and recovery of mCherry-MT1-MMP fluorescence was recorded for 58 s. (B) Quantification of fluorescence recovery was of at least 30 cells in three independent experiments. Error bars indicate means ± SEM. Bar, 10 µm.