Figure 5.
Figure 5. N-WASP is crucial for pericellular collagenolysis in vitro and on native BMs. (A) Immunofluorescence images of cells on FITC-conjugated type-I collagen film (green). Cells were labeled for filamentous actin (red, phalloidin) and DNA (blue, DAPI). Bar, 50 µm. (B) Quantification of FITC fluorescence release after incubating cells with DQ collagen matrix from three independent experiments. (C) MDA-MB-231 cells were cultured atop of mouse peritoneal BM for 3 d and then fixed and stained for collagen IV (green), actin (phalloidin, red), and DNA (DAPI, blue). The cells remodel and degrade BM, and the remaining collagen IV is shown by antibody staining (green). Bar, 100 µm. (D) The total fluorescence intensity of remaining collagen IV is represented in the bar graph. At least three independent experiments were performed and quantified. Error bars indicate means ± SD; **, P < 0.01; *, P < 0.05 by t test.

N-WASP is crucial for pericellular collagenolysis in vitro and on native BMs. (A) Immunofluorescence images of cells on FITC-conjugated type-I collagen film (green). Cells were labeled for filamentous actin (red, phalloidin) and DNA (blue, DAPI). Bar, 50 µm. (B) Quantification of FITC fluorescence release after incubating cells with DQ collagen matrix from three independent experiments. (C) MDA-MB-231 cells were cultured atop of mouse peritoneal BM for 3 d and then fixed and stained for collagen IV (green), actin (phalloidin, red), and DNA (DAPI, blue). The cells remodel and degrade BM, and the remaining collagen IV is shown by antibody staining (green). Bar, 100 µm. (D) The total fluorescence intensity of remaining collagen IV is represented in the bar graph. At least three independent experiments were performed and quantified. Error bars indicate means ± SD; **, P < 0.01; *, P < 0.05 by t test.

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