N-WASP expression increases in both human and mouse invasive breast cancers. (A) Immunohistochemical staining of N-WASP in human breast cancer tissues. Left and middle panels show expression of N-WASP in human normal tissue and DCIS. The right panel shows the expression level of N-WASP in human invasive ductal carcinoma. The insets show enlarged images of N-WASP staining in different tissues. (B) Bar graphs indicate histochemistry scores of normal/DCIS and invasive breast cancer cores from a 75-core human breast cancer TMA. All error bars indicate means ± SD; *, P < 0.05 by t test. (C) Immunohistochemical staining of N-WASP in mouse virgin and lactating mammary glands. Samples from three mice were stained and imaged. The insets show enlarged images of N-WASP staining in mouse virgin and lactating mammary glands. (D) Immunohistochemical staining of N-WASP in primary tumors from MMTV-PyMT (n = 9) and MMTV-erbB2 (n = 5) mouse models. Bars: (main panels) 200 µm; (insets) 100 µm.