Disrupting the endocytic signal prevents VE-cadherin recruitment into clathrin-enriched membrane domains. (A) COS-7 cells expressing wild-type (WT), DEE mutant, or GGG mutant IL-2R–VE-cadherin (VE-cad) cytoplasmic tail chimeras were surface labeled with an antibody against IL-2R. After a 30-min incubation on ice to allow chimera clustering, the cells were fixed and stained for clathrin heavy chain. Boxes show inset areas. Bar, 10 µm. (B) Quantification of IL-2R–VE-cadherin chimera and clathrin colocalization. Pearson’s correlation coefficient; means ± SEM (n = 33–34 cells per group); *, P < 0.05 compared with wild type and GGG mutant.