Figure 8. VE-cadherin mobility does not... | Rockefeller University Press

Figure 8.

$Figure 8. VE-cadherin mobility does not require endocytosis. RFP-tagged wild-type (WT), DEE mutant, or GGG mutant VE-cadherin (VE-cad) was expressed in primary human dermal microvascular endothelial cells and used in FRAP experiments. (A) False-color images of VE-cadherin–RFP at cell junctions before bleaching (left), immediately after bleaching a 5-µm-long section of the junction (indicated by arrowheads), and at 5 and 10 min after bleaching. See also Videos 3, 4, and 5. Bar, 5 µm. (B) Quantification of fluorescence recovery. Mean fluorescence within the bleach area, corrected for image acquisition–related photobleaching, as a fraction of prebleach value. Exponential curves were fit to the data, and their coefficients are given in Table 1. Points, means ± SEM (n = 16–18 sequences per group); lines, exponential models ± 95% confidence interval.$

VE-cadherin mobility does not require endocytosis. RFP-tagged wild-type (WT), DEE mutant, or GGG mutant VE-cadherin (VE-cad) was expressed in primary human dermal microvascular endothelial cells and used in FRAP experiments. (A) False-color images of VE-cadherin–RFP at cell junctions before bleaching (left), immediately after bleaching a 5-µm-long section of the junction (indicated by arrowheads), and at 5 and 10 min after bleaching. See also Videos 3, 4, and 5. Bar, 5 µm. (B) Quantification of fluorescence recovery. Mean fluorescence within the bleach area, corrected for image acquisition–related photobleaching, as a fraction of prebleach value. Exponential curves were fit to the data, and their coefficients are given in Table 1. Points, means ± SEM (n = 16–18 sequences per group); lines, exponential models ± 95% confidence interval.