p120 binding is not required for adherens junction assembly. (A–D) Wild-type (WT), DEE mutant, or GGG mutant VE-cadherin (VE-cad) with a C-terminal RFP tag was expressed in A-431D cells, which lack endogenous cadherins, using an adenoviral system. Cells were fixed and stained for VE-cadherin and α-catenin (A), β-catenin (β-cat; B), p120 (C), or actin (D). (E) Wild-type or mutant VE-cadherin–RFP was expressed in primary human microvascular endothelial cells. Cells were fixed and stained for RFP (VE-cadherin), actin, and PECAM-1. Bars: (A–D) 5 µm; (E) 20 µm.