Tiam1 and talin together control adhesion turnover for polarized cell migration. (A) U251 cells were transfected with the indicated siRNAs and EGFP-paxillin. 18 h after seeding onto FN, EGFP-paxillin dynamics was monitored at 5-min intervals for 5 h. Broken and white circles indicate dynamic and static adhesions, respectively. Bars, 10 µm. (B) Apparent rate constants for assembly (left) and rate constant for disassembly (right). Results are representative of more than three independent experiments.