Figure 1. Talin is a novel... | Rockefeller University Press

Figure 1.

$Figure 1. Talin is a novel Tiam1-interacting protein. (A) Schematic diagram of Tiam1. The domain organization of Tiam1 and its fragments is represented. (B) The cytoplasmic fraction of a porcine brain homogenate was loaded onto beads coated with GST, GST-Tiam1-PHnCCEX, or GST-Tiam1-PDZ4. Aliquots of the eluate were resolved by SDS-PAGE, followed by silver staining (bottom) or immunoblotting with anti-talin antibody (top). (C) Lysates of COS7 cells expressing both EGFP-talin1 and Tiam1-HA were precipitated with anti-GFP or anti-talin antibody. Tiam1 was coprecipitated with talin by both antibodies. (D) The lysates of U251 cells were precipitated with indicated antibodies, followed by silver staining (right) and immunoblotting (left). Silver staining revealed a specific band pattern in each antibody. Endogenous talin was specifically coprecipitated with Tiam1. Results are representative of more than three experiments.$

Talin is a novel Tiam1-interacting protein. (A) Schematic diagram of Tiam1. The domain organization of Tiam1 and its fragments is represented. (B) The cytoplasmic fraction of a porcine brain homogenate was loaded onto beads coated with GST, GST-Tiam1-PHnCCEX, or GST-Tiam1-PDZ4. Aliquots of the eluate were resolved by SDS-PAGE, followed by silver staining (bottom) or immunoblotting with anti-talin antibody (top). (C) Lysates of COS7 cells expressing both EGFP-talin1 and Tiam1-HA were precipitated with anti-GFP or anti-talin antibody. Tiam1 was coprecipitated with talin by both antibodies. (D) The lysates of U251 cells were precipitated with indicated antibodies, followed by silver staining (right) and immunoblotting (left). Silver staining revealed a specific band pattern in each antibody. Endogenous talin was specifically coprecipitated with Tiam1. Results are representative of more than three experiments.

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