Targeting GFP-NuMA(1–705) to the plasma membrane recruits dynein to that location. (A–D) Untransfected control cells (A and C) or cells transfected with GFP-NuMA(1–705)-CAAX (B and D) and stained for the dynactin subunit p150^Glued (A and B) or the DIC (C and D) as well as GFP. Arrows point to cortical p150^Glued or DIC signals, and arrowheads point to spindle pole p150^Glued. 50 cells were scored for each condition. Note the presence of GFP in the vicinity of spindle poles (B), perhaps owing to the presence of endomembranes near spindle poles. (E–G) HeLa cells transfected with control (scrambled siRNAs; E), siRNAs against NuMA (F), or HeLa cell treated with siRNAs against NuMA and also transfected with GFP-NuMA(1–705)-CAAX (G) and stained for GFP (left) and p150^Glued (right). The bar graphs on the right represent the percentage of cells with clear cortical p150^Glued signal from two independent experiments along with SEM. Arrows point to cortical p150^Glued, and arrowheads point to p150^Glued at spindle poles. More than 50 cells were scored for each condition in two independent experiments.