Figure 4.
Figure 4. The G2 and prolonged interphase after pairwise cyclin knockdown requires the replication checkpoint. (A) Inactivation of Chk1 (grp−) or deletion of S phase (by Geminin injection) shortened interphase in control and cyclin RNAi-treated embryos and reduced the differences among cyclin types. Error bars represent SDs. Detailed measurements are listed in Table S1. Data for cyclin RNAi experiments in wild-type embryos are reproduced from Fig. 1 D for purpose of comparison. (B–E) Video frames of grp− mutant embryos in cycle 13 (GFP-PCNA is shown in white, and histone-RFP is shown in red) aligned at the start of DNA condensation (t = 00:00). Time is given in minutes and seconds. Bars, 5 µm. (F) A schematic model in which checkpoint inhibition of cyclin–Cdk1 (gray) is reversed by compartment-specific action of cyclins plus a slow communication between compartments (arrows).

The G2 and prolonged interphase after pairwise cyclin knockdown requires the replication checkpoint. (A) Inactivation of Chk1 (grp−) or deletion of S phase (by Geminin injection) shortened interphase in control and cyclin RNAi-treated embryos and reduced the differences among cyclin types. Error bars represent SDs. Detailed measurements are listed in Table S1. Data for cyclin RNAi experiments in wild-type embryos are reproduced from Fig. 1 D for purpose of comparison. (B–E) Video frames of grp− mutant embryos in cycle 13 (GFP-PCNA is shown in white, and histone-RFP is shown in red) aligned at the start of DNA condensation (t = 00:00). Time is given in minutes and seconds. Bars, 5 µm. (F) A schematic model in which checkpoint inhibition of cyclin–Cdk1 (gray) is reversed by compartment-specific action of cyclins plus a slow communication between compartments (arrows).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal