Culture systems to analyze lumen formation using MDCK cells. Description of MDCK cell culture systems. The table summarizes cell support, bottom ECM coating, and culture medium and provides information on cell spreading behavior and lumen formation in each condition. The right column shows a scheme of an x-z section of each culture system, indicating position of cells and apical membranes in different ECM supports. Additionally, in micropattern culture systems, an x-y view of micropatterns and their different sizes is also shown.