Glu-MTs are necessary for MTOC reorientation. (A and B) Glu-MT formation and MTOC distribution in Jurkat cells conjugated with latex beads coated with either anti-transferrin receptor (TfR) or anti-TCR antibodies in the presence or the absence of 2.5 µM PP2 (A). The histogram represents the percentage of T cells with Glu-MTs or with polarized MTOC (B). (C–E) Jurkat cells coexpressing GFP and shINF2b were transfected or not transfected with a DNA construct expressing the intact FH2 domain of INF2, as indicated. Cells were then conjugated to latex beads coated with either anti-TfR or anti-TCR antibodies and were stained for the expressed INF2 FH2 fragment and Glu-MT (C) or α-tubulin (D), as indicated. INF2-KD cells treated with 3 nM taxol for 18 h were activated with anti-TfR or anti-TCR–coated beads, and analyzed for MTOC reorientation by staining for α-tubulin (E). The histogram represents the percentage of T cells with Glu-MTs or polarized MTOC (E). Bars, 5 µm. The arrowheads indicate the position of the MTOC. At least 40 T cells were analyzed in B and E. Data in B and E are summarized as means ± SEM from three independent experiments (error bars; *, P < 0.05; **, P < 0.01; ***, P < 0.001).