Subdendritic translocation of αCaMKII correlates with an increase of surface GluA1 at neighboring postsynaptic sites. (A) Dendrite coexpressing mCherry-αCaMKII and SEP-GluA1 stimulated with 0Mg²⁺/Gly for 5 min. Brackets indicate CaMKII dendritic hot spots (D+, white) or adjacent dendritic region (D−, red). Arrows point to a spine near (1) or adjacent (2) to an mCherry-αCaMKII dendritic hot spot. (n = 7 neurons, 24–43 synapses per group). Bar, 5 µm. (B) Representative examples of SEP-GluA1 intensity at postsynaptic sites for synapses without (blue, S−) or with (S+) synaptic CaMKII translocation, which are near (black, S+D+) or not (red, S+D−) a CaMKII dendritic hot spot. Synapses 1 and 2 are pointed in A. Bar, 0.5 µm. (C–E) Time-lapse analysis (C), histogram (D), and cumulative distribution (E) of the increase in SEP-GluA1 for synapses in the same category as described in B. *, P < 0.05 Anova followed by Tukey’s least-significant difference test.