Subdendritic translocation of αCaMKII is associated with a localized increase in CaMKII activity. (A) Dendrite immunolabeled for endogenous αCaMKII and phospho-T286 CaMKII in high Mg²⁺ solution or after a 5-min stimulation with 0Mg²⁺/Gly. Brackets indicate CaMKII dendritic hot spots (D+, white) or adjacent dendritic region (D−, red). (B) Analysis of pT286/αCaMKII ratio in dendritic regions of neurons as in A. n = 40 neurons per condition. *, P < 0.05 Kruskal-Wallis followed by Tukey’s least-significant difference test. (C) Depolarized dendrite exhibits phospho-T286 CaMKII on fiber-like structures. Bars, 5 µm.