Figure 4.
Figure 4. Localization of MDs. (A) Western blot analysis of wild-type (wt), ida4, ida5, ida10, and pf3 axonemes using antibodies to DHC3, DHC4, DHC11, and IC140 (an IC of dynein f, used as a positive control). The molecular masses of DHC3, DHC4, and DHC11 are expected values, as they migrate significantly more slowly than the 250-kD molecular mass marker, which is not shown. (B) The 96-nm surface renderings of MTD2-8+ and MTD1+ of wild type and MTD2-9− and MTD1− of wild type, ida4, ida5, ida10, ida1, and pf3. There are three MD densities that deviated from the common inner-arm dynein structures, MD1–3, indicated with arrowheads in different colors. They can be the candidates of the MDs. A filled arrowhead indicates the presence of dynein, whereas an open arrowhead with the same border color indicates the absence of dynein from the respective position. Bar, 16 nm.

Localization of MDs. (A) Western blot analysis of wild-type (wt), ida4, ida5, ida10, and pf3 axonemes using antibodies to DHC3, DHC4, DHC11, and IC140 (an IC of dynein f, used as a positive control). The molecular masses of DHC3, DHC4, and DHC11 are expected values, as they migrate significantly more slowly than the 250-kD molecular mass marker, which is not shown. (B) The 96-nm surface renderings of MTD2-8+ and MTD1+ of wild type and MTD2-9− and MTD1− of wild type, ida4, ida5, ida10, ida1, and pf3. There are three MD densities that deviated from the common inner-arm dynein structures, MD1–3, indicated with arrowheads in different colors. They can be the candidates of the MDs. A filled arrowhead indicates the presence of dynein, whereas an open arrowhead with the same border color indicates the absence of dynein from the respective position. Bar, 16 nm.

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