Loss of KIF4A decreases MT dynamics. (A) HeLa cells were transfected with KIF4A RNAi or mock, stained for EB1 and kinetochores (CREST antiserum) and imaged by confocal microscopy. Representative maximum intensity projections are shown. Bar, 10 µm. (B) HeLa EB1-GFP cells were transfected with either KIF4A siRNA or mock and imaged 36 h pt. MT plus-ends, marked by EB1, were followed over several frames and MT length and stability were calculated. Bar, 5 µm. Shown is mean of three independent experiments ± SEM.