Loss of KIF4A increases sister separation and prolongs the breathing period. (A) Kinetochore distribution across a fitted metaphase plate was determined by measuring the sister center position and kinetochore positions. Both values are displayed as the twofold standard deviation of the mean ± SD. (B and C) Dynamic parameters were determined by calculating the sister displacement cross-correlation (B) and sister kinetochore autocorrelation values (C). Methanol-fixed wild-type cells (gray, dashed line) were used as negative control. (D) Oscillation and breathing speed for different siRNA treatments. Columns represent mean + SD. (E) Table summarizing the kinetochore oscillation parameters. *, Student’s t test value P < 0.05; **, P < 0.01; ***, P < 0.001.