Figure 3.
Figure 3. HCT116 KIF4A knockout cells show mitotic delay after depletion of hKID. (A) Selected spindles of HCT116 wild-type and KIF4A KO cells after treatment with mock or hKID-siRNA. Shown are MIPs of deconvolved confocal z-stacks. Arrows indicate aberrant nonkinetochore MTs in KIF4A KO cells. Bar, 5 µm. (B) HCT116 wild-type or KIF4A KO cells were transfected with 50 nM mock or hKID-siRNA, methanol-fixed 40 h pt, and stained for DNA. Mitotic index and mitotic phases of four independent experiments + SEM are shown in B and C, respectively. *, Student’s t test value P < 0.05; **, P < 0.01.

HCT116 KIF4A knockout cells show mitotic delay after depletion of hKID. (A) Selected spindles of HCT116 wild-type and KIF4A KO cells after treatment with mock or hKID-siRNA. Shown are MIPs of deconvolved confocal z-stacks. Arrows indicate aberrant nonkinetochore MTs in KIF4A KO cells. Bar, 5 µm. (B) HCT116 wild-type or KIF4A KO cells were transfected with 50 nM mock or hKID-siRNA, methanol-fixed 40 h pt, and stained for DNA. Mitotic index and mitotic phases of four independent experiments + SEM are shown in B and C, respectively. *, Student’s t test value P < 0.05; **, P < 0.01.

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