Clathrin inactivation or inhibition of Aurora A kinase reduces clathrin and ch-TOG at centrosomes. (A) Clone 3.3 cells were synchronized by T/T block and treated at T0 with BG-GLA-BG for 2 h or the Aurora A kinase inhibitor MLN8054 for 8 h. 8 h after T0, cells were fixed in methanol and processed for IF to visualize clathrin (mAb X16), PCNT2, and ch-TOG. Three-color colocalization in merge is white, and red-blue colocalization in merge is pink. Bars, 5 µm. (insets) Boxed regions are magnified. Bars, 2 µm. (B) Quantification by Pearson’s coefficient of overlap between the centrosomal marker PCNT2 and clathrin or ch-TOG for each treatment in A. Data represent the mean ± SEM from two independent experiments (**, P < 0.01; ***, P < 0.001).
