Depletion of either CHC17 or CHC22 increases multinucleation. (A) HeLa-GFP–α-tubulin cells were treated (48 h) with siRNA, as indicated (top). Lysates of treated cells were immunoblotted for the proteins indicated (left). Molecular mass marker position is shown on the right. (B) The percentage of binuclear and multinuclear cells was assessed at 48 or 72 h after each siRNA treatment indicated by nuclear staining with DAPI (>100 cells per treatment per experiment were scored). Targeted siRNA effects were compared with nonsilencing siRNA (siNonsilencing) effects for statistical significance. The mean ± SD of four to five independent experiments is shown (*, P < 0.1).