Figure 2.
Figure 2. Centrosome fragmentation is observed in early mitotic cells depleted for CHC17 and CLCs. HeLa-GFP–α-tubulin cells treated (72 h) with siRNA, as indicated (left), were processed for IF to detect PCNT2 and clathrin (CHC17). GFP–α-tubulin was visualized in green. Red-green merge is yellow. Centrosome fragments, defined by PCNT2 staining, that are not localized to a spindle pole are indicated (arrowheads). Bar, 7.5 µm. (insets) Boxed regions are magnified threefold and highlight more diffuse PCNT2 staining at spindle poles in clathrin-depleted samples compared with the nonsilencing siRNA (siNonsilencing) treatment. Bar, 2 µm. All images are 2D.

Centrosome fragmentation is observed in early mitotic cells depleted for CHC17 and CLCs. HeLa-GFP–α-tubulin cells treated (72 h) with siRNA, as indicated (left), were processed for IF to detect PCNT2 and clathrin (CHC17). GFP–α-tubulin was visualized in green. Red-green merge is yellow. Centrosome fragments, defined by PCNT2 staining, that are not localized to a spindle pole are indicated (arrowheads). Bar, 7.5 µm. (insets) Boxed regions are magnified threefold and highlight more diffuse PCNT2 staining at spindle poles in clathrin-depleted samples compared with the nonsilencing siRNA (siNonsilencing) treatment. Bar, 2 µm. All images are 2D.

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