Localization and level of expression of the split kinesin components. (A and B) A cell co-expressing a split kinesin tail (FRB-myc-KIF13B tail) and the KIF5C⁵⁵⁹-tdTM-FKBP motor domain. Immunostaining for the kinesin tail was present throughout the cell body and dendrites and extended far into the axon (arrows). The motor domain was concentrated at the tips of axonal branches (arrowheads) but not at dendritic tips (asterisks). (C) Comparison of the expression levels of the different split kinesin tails (based on anti-myc immunostaining). Average intensity of staining in the cell body was measured for at least 15 cells from two separate experiments in each condition. Bar, 20 µm.