Mss4 enrichment to small buds and hyphal filament tips requires the actin cytoskeleton. (A) F-actin is critical for asymmetric distribution of Mss4 in budding cells. A mss4 strain expressing GFP-Mss4 was grown with Dox in the presence or absence of LatA as in Fig. 6 A. Central z-sections and sum projections (10 z-sections) of representative cells from different fields of view are shown (top) and quantification of Mss4 concentration over long axis of cells (bottom) as in Fig. 4 A. (B) F-actin is required for asymmetric Mss4 distribution in germ tubes. A mss4 strain expressing GFP-Mss4 was treated with FCS and then incubated with LatA as in Fig. 6 B. Images as described in Fig. 9 A (top) and quantification of Mss4 concentration over long axis of cells (bottom) as in Fig. 4 A (n = 50 cells). For Fig. 9 (A and B), ni.ex. = 3.
