Ags1 localizes in the growing sites during vegetative and sexual phases. (A) Predicted Ags1 topology and tested GFP insertions to obtain a functional Ags1-GFP (see Materials and methods). (B) Physiological Ags1-GFP localization throughout the cell cycle. (C) Magnification of Ags1-GFP localization in the CAR and septum membrane. (D and E) Ags1 is localized to all sites of wall synthesis during sexual differentiation (D) and spore germination (E). h90 ags1⁺-GFP cells were grown to early stationary phase and transferred onto SPA plates. (D) Samples were collected after 3, 5, 8, 24, and 48 h and examined. (E) Spores were collected after 10 d and incubated in YES medium. Samples were taken after 2 to 11 h. Bars, 2.5 µm.