Insulin recruits Rab10-marked GSVs to the PM. EGFP-labeled Rab4A (A), Rab8A (B), Rab10 (C), and Rab14 (D) were separately transfected into adipocytes together with GLUT4-mCherry, and their insulin responsiveness was followed using dual-color TIRF microscopy. The first two columns in C are enlarged and displayed in E and F. Representative vesicles positive for both GLUT4 and Rab10 are indicated with arrowheads in F. Bars, 4 µm. (G) Rab10 gets activated and attaches to GSV before the vesicle gets into the TIRF zone. Adipocytes were cotransfected with EGFP-Rab10 and GLUT4-mCherry, and images were taken 3 min after insulin stimulation. Vesicle intensities measured from both channels are plotted on the right, and the docking stage is indicated with horizontal lines. Bars, 1 µm.