Figure 1. Motility of nuclear pores in... | Rockefeller University Press

Figure 1.

$Figure 1. Motility of nuclear pores in fungi. (A) Freeze-fracture electron micrograph (top) and Nup107-GFP (N107; bottom) showing even distribution of NPCs in the nuclear envelope. Bars indicate micrometers. See also Video 1. (B) Image series showing directed motility of a NPC within the nuclear envelope. Bottom left image shows the nucleus from where the image series was taken (red box); the edge of the nucleus is indicated by a blue dotted line. Contrast was inverted. Time is given in seconds; bar indicates micrometers. See also Video 2. (C) Kymographs showing motility behavior of Nup107-GFP–labeled NPCs (N107). Rapid motility is indicated by arrowheads. Occasionally, several pores show coordinated motility (bottom). Contrast was inverted. Bars represent micrometers and seconds. See also Video 2. (D) Colocalization of Nup107-GFP (N107) and a nuclear reporter protein (nRFP). A NPC is pulled away from the nucleus, which extends the nucleus (arrowhead). See also Videos 3 and 4. (E) Kymographs showing motility behavior of NPCs in the budding yeast S. cerevisiae (labeled with Nup82-GFP) and the filamentous fungus A. nidulans (labeled with Nup133-GFP). Contrast was inverted. Bars represent micrometers and seconds. See also Videos 5 and 6. (F) Bar chart showing velocity of NPC motility in U. maydis, S. cerevisiae, and A. nidulans. One-way ANOVA testing showed no significant difference (P = 0.112). Bars represent mean ± SEM (n = 55–65 measurements per bar).$

Motility of nuclear pores in fungi. (A) Freeze-fracture electron micrograph (top) and Nup107-GFP (N107; bottom) showing even distribution of NPCs in the nuclear envelope. Bars indicate micrometers. See also Video 1. (B) Image series showing directed motility of a NPC within the nuclear envelope. Bottom left image shows the nucleus from where the image series was taken (red box); the edge of the nucleus is indicated by a blue dotted line. Contrast was inverted. Time is given in seconds; bar indicates micrometers. See also Video 2. (C) Kymographs showing motility behavior of Nup107-GFP–labeled NPCs (N107). Rapid motility is indicated by arrowheads. Occasionally, several pores show coordinated motility (bottom). Contrast was inverted. Bars represent micrometers and seconds. See also Video 2. (D) Colocalization of Nup107-GFP (N107) and a nuclear reporter protein (nRFP). A NPC is pulled away from the nucleus, which extends the nucleus (arrowhead). See also Videos 3 and 4. (E) Kymographs showing motility behavior of NPCs in the budding yeast S. cerevisiae (labeled with Nup82-GFP) and the filamentous fungus A. nidulans (labeled with Nup133-GFP). Contrast was inverted. Bars represent micrometers and seconds. See also Videos 5 and 6. (F) Bar chart showing velocity of NPC motility in U. maydis, S. cerevisiae, and A. nidulans. One-way ANOVA testing showed no significant difference (P = 0.112). Bars represent mean ± SEM (n = 55–65 measurements per bar).