KASH5 localizes at telomeres with SUN1 during meiotic prophase. (A) Spermatocytes were cultured with EdU and stained with Alexa Flour 488 azide and antibodies against SCP3 and KASH5 (left) or SUN1 (right). L, leptotene; P, pachytene. (B) Chromosome spreads from spermatocytes were stained with KASH5 and SCP3 antibodies. (C) Chromosome spreads from spermatocytes were stained with KASH5, SUN1, and SCP3 antibodies. Bars, 5 µm (unless otherwise indicated).