Figure 2.
Figure 2. K-Ras V12 expression disrupts apical polarity and tight junctions and promotes hyperproliferation. (A) Day 10 3D cultures expressing control (pQCXIP GFP) or K-Ras V12 (pQCXIP GFP K-Ras V12) were lysed and analyzed by Western blotting with the indicated antibodies. (B) Day 1, 4, 7, and 10 3D cultures expressing control (pQCXIP GFP) or K-Ras V12 (pQCXIP GFP K-Ras V12) were fixed and stained with actin and DNA. The number of nuclei in the midplane of ≥50 structures was determined for each condition in three independent experiments. The means ± SEM are shown. *, P < 0.05; **, P < 0.005; ***, P < 0.0005. (C–F) Day 10 3D structures expressing control (pQCXIP GFP) or K-Ras V12 (pQCXIP GFP K-Ras V12) were fixed and stained for Ki67, aPKC, and DNA (blue; C); aPKC, actin, and DNA (blue; D); ezrin, laminin, and DNA (blue; E); and ZO-1, E-cadherin, and DNA (blue; F). Representative images are shown. Bars, 50 µm.

K-Ras V12 expression disrupts apical polarity and tight junctions and promotes hyperproliferation. (A) Day 10 3D cultures expressing control (pQCXIP GFP) or K-Ras V12 (pQCXIP GFP K-Ras V12) were lysed and analyzed by Western blotting with the indicated antibodies. (B) Day 1, 4, 7, and 10 3D cultures expressing control (pQCXIP GFP) or K-Ras V12 (pQCXIP GFP K-Ras V12) were fixed and stained with actin and DNA. The number of nuclei in the midplane of ≥50 structures was determined for each condition in three independent experiments. The means ± SEM are shown. *, P < 0.05; **, P < 0.005; ***, P < 0.0005. (C–F) Day 10 3D structures expressing control (pQCXIP GFP) or K-Ras V12 (pQCXIP GFP K-Ras V12) were fixed and stained for Ki67, aPKC, and DNA (blue; C); aPKC, actin, and DNA (blue; D); ezrin, laminin, and DNA (blue; E); and ZO-1, E-cadherin, and DNA (blue; F). Representative images are shown. Bars, 50 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal