Cortical For2A linear movement is actin dependent. (A) Two representative cells imaged with VAEM show that For2A-GFP localizes to cortical dots (left images) and Lifeact-mCherry labels the cortical F-actin array (center images). For2A linear trajectories are apparent in a maximum projection of 30 frames, which corresponds to three seconds real time (right panels). See also Video 8. (B) Quantification of the percentage of cortical dots found in the three categories of trajectories identified for formin cortical dots that resided on the cortex for more than 1 s. Stationary indicates that no movement greater than 0.4 µm from the origin was observed. (C) Two representative cells imaged with VAEM in the presence of 16 µM latrunculin B show that For2A-GFP localizes to cortical dots (left images) but F-actin has been disrupted, depicted by diffuse Lifeact-mCherry fluorescence (center images). Furthermore, For2A linear trajectories were no longer observed in maximum projections of 30 frames (right panels). See also Video 8. Bars, 5 µm.