Figure 5.
Figure 5. PI(3,5)P2 binding is essential for For2A function in vivo. (A) Representative chlorophyll autofluorescence images of control RNAi (GUS-RNAi), For2 RNAi (For2AB-5′UTR), and For2 RNAi plants co-transformed with indicated constructs. Bar, 100 µm. (B) Quantification of area and circularity by chlorophyll autofluorescence shows that replacing the For2A PTEN domain with nonhomologous domains from various organisms exclusively rescues For2 RNAi when the domain binds PI(3,5)P2, indicating that PI(3,5)P2 binding is essential for class II formin function in polarized growth. Number of plants analyzed: 175, GUS-RNAi; 176, For2AB-5′UTR; 100, +For2A; 75, +2XFYVE-FH1FH2-3XFLAG; 50, +TAPP1-FH1FH2-3XFLAG; 25, +PH-FH1FH2-3XFLAG; 78, +ATG18-FH1FH2; 75, +MTM1-FH1FH2; 78, +MTM1*-FH1FH2. Error bars represent SEM and letters above the bars indicate statistical groups with α = 0.05 using an ANOVA analysis.

PI(3,5)P2 binding is essential for For2A function in vivo. (A) Representative chlorophyll autofluorescence images of control RNAi (GUS-RNAi), For2 RNAi (For2AB-5′UTR), and For2 RNAi plants co-transformed with indicated constructs. Bar, 100 µm. (B) Quantification of area and circularity by chlorophyll autofluorescence shows that replacing the For2A PTEN domain with nonhomologous domains from various organisms exclusively rescues For2 RNAi when the domain binds PI(3,5)P2, indicating that PI(3,5)P2 binding is essential for class II formin function in polarized growth. Number of plants analyzed: 175, GUS-RNAi; 176, For2AB-5′UTR; 100, +For2A; 75, +2XFYVE-FH1FH2-3XFLAG; 50, +TAPP1-FH1FH2-3XFLAG; 25, +PH-FH1FH2-3XFLAG; 78, +ATG18-FH1FH2; 75, +MTM1-FH1FH2; 78, +MTM1*-FH1FH2. Error bars represent SEM and letters above the bars indicate statistical groups with α = 0.05 using an ANOVA analysis.

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