Dynamics of For2A and actin in a growing tip cell. (A) For2A-GFP is present in the apex and Lifeact-mCherry is present subapically mostly along the cell cortex. The majority of the time, For2A-GFP and Lifeact-mCherry do not overlap. Occasionally a short overlap followed by quick actin polymerization can be seen (25–35 min). See also Video 4. (B) Away from the tip, bursts of For2A-GFP are observed, followed by actin polymerization near the For2A-GFP enrichment. Here, the For2A-GFP cloud travels through the cell with actin filaments behind it. See also Video 5. Bars, 5 µm.